Enzyme-linked immunosorbent assay for hyaluronate using cartilage proteoglycan and an antibody to keratan sulfate

✍ Scribed by Ronald L. Goldberg

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 895 KB
• Volume: 174
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(88)90043-7

✦ Synopsis

An enzyme-linked immunosorbent assay has been developed to measure hyaluronate concentrations in biological samples. The assay is based on the aggregation of hyaluronate with cartilage proteoglycan monomer, followed by binding of a monoclonal antibody to the keratan sulfate on the proteoglycan. The sensitivity of the assay is 10 ng hyaluronate/ml of either serum or conditioned cell culture medium. The coefficient of variability was between 10 and 20%. Hyaluronate added to samples was recovered quantitatively and digestion of cell culture medium with protease did not affect the concentration of hyaluronate. Hyaluronate polysaccharides as small as a decasaccharide can be measured. This sensitive and convenient assay can be used for measuring large numbers of biological samples from a variety of animal and tissue sources.