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Sensitive enzyme-linked immunosorbent assay for antibody to varicella-zoster virus using purified VZV glycoprotein antigen

✍ Scribed by Edward H. Wasmuth; William J. Miller

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
510 KB
Volume
32
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

The development of an ELISA of increased sensitivity has permitted a more critical evaluation of human humoral immune responses to the live attenuated varicella (Oka/Merck) vaccine. For use as a solid‐phase antigen, the glycoprotein (gp) antigens are prepared by lectin‐affinity chromatography from lysates of VZV‐infected MRC‐5 cells. The lot‐to‐lot variation in VZV gp content is controlled by standardization of antigen against a panel of human serum providing antigencoated plates of consistent quality. The increased sensitivity of the gpELISA over the VAR ELISA is reflected in the greater seroconversion rate and prepositive rate specificity. These determinations have been shown to be specific for anti‐VZV by absorption experiments using purified VZV gp antigens.

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