Enzyme channeling immunoassay: A new hom
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David J. Litman; Teresa M. Hanlon; Edwin F. Ullman
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Article
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1980
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Elsevier Science
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English
โ 568 KB
A homogeneous enzyme immunoassay has been developed in which an antigen and glucose-6-phosphate dehydrogenase are coimmobilized on agarose beads. Binding of hexokinase-labeled antibody to the bead-bound antigen results in an accelerated conversion of glucose, ATP, and NAD+ to 6-phosphogluconolactone