Enzyme-amplified immunoassays
โ Scribed by C.J. Stanley; D.H. Ellis; D.L. Bates; A. Johannsson
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 478 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0731-7085
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Methods for removing contammatmg FAD and alkahne phosphatase from apo-ammoaad oxldase are described A method for phosphorylatmg flavm ademne dmucleotlde is also described Its hydrolysis to flavm ademne dmucleotlde catalysed by alkahne phosphatase IS demonstrated by hqmd chromatography Flavm ademne d
An immunometric assay is described which allows fast detection of attomole amounts of an antigen. The sensitivity is 100 to 1000 times better than that of classical sandwich immunometric assays. Our system allowed the measurement of human growth hormone in the range of 0.1 amol to 100 fmol in a 4-h
## AhtraCt A competitive enzyme amplified ELBA for steroids was developed using recycling of NADH/NAD+ between the enzymes diaphorase and alcohol dehydrogenase. The substrate was generated from the steroid-bound enzyme label alkaline phosphatase, which dephosphorylated NADPH or NADP+. Secondary an
A homogeneous enzyme immunoassay has been developed in which an antigen and glucose-6-phosphate dehydrogenase are coimmobilized on agarose beads. Binding of hexokinase-labeled antibody to the bead-bound antigen results in an accelerated conversion of glucose, ATP, and NAD+ to 6-phosphogluconolactone