✦ LIBER ✦

Microtitre plate enzyme amplified immunoassay for thyroid stimulating hormone

✍ Scribed by Robert Wilson

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 485 KB
Volume: 269
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(92)85416-4

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✦ Synopsis

Methods for removing contammatmg FAD and alkahne phosphatase from apo-ammoaad oxldase are described A method for phosphorylatmg flavm ademne dmucleotlde is also described Its hydrolysis to flavm ademne dmucleotlde catalysed by alkahne phosphatase IS demonstrated by hqmd chromatography Flavm ademne dmucleotlde can reactivate apo-ammoacld oxldase and result m the formatlon of hydrogen peroxlde The hydrogen peroxide can be detected colonmetrlcally These reactlons are used m an enzyme amplified immunoassay for thyroid stlmulatmg hormone m which alkaline phosphatase IS the enzyme label A hmlt of detection of 0 03 pIU ml-' for thyroid stimulating hormone makes the assay suitable for dlagnosmg hyperthyroldlsm Keywords Immunoassay, Enzyme amplified mununoassay, Flavm ademne dmucleotlde phosphate, Mlcrotltre plate, Nitroethane, Suicide substrate, Thyroid stimulatmg hormone

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