Enzyme immunoassay for c-reactive protein analysis

✍ Scribed by Sharon K. Unten; Y. Hokama

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 334 KB
• Volume: 1
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860010210

✦ Synopsis

A solid-phase enzyme immunoassay yr), 1,145 f 116; adults (36-50 yr), method using goat anti-CRP sensitized to 3,775 f 605; and adults (51-83 yr), polyvinyl 96 well plates and a second rab-7,718 f 1,482. Individuals in the age range bit anti-CRP highly specific for gamma mo-of 22-34 yr with one or more abnormality bility CRP is described in this study. in the chemistry profile analysis showed Examination of serum samples of normal CRP levels with a higher x f S.E.M. healthy individuals from cord blood, chil-(3,615 f 925). This is in contrast to the dren (2-12 yr) and adults (16-83 yr) by this normal individuals of the same age range EIA method showed a progressive in- (1,145 +_ 116). This EIA method is sensicrease in CRP levels with age. The mean tive, reproducible, and specific. It should and standard error of the mean in nglml be a useful quantitative method for moni-CRP was as follows with respect to age toring low level CRP responders, such as distribution: cord blood, 310 f 54; chil-newborn and cord blood serum. dren (2-12 yr), 664 f 87; adults (16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)