Thermolysin-catalyzed peptide synthesis
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Hiromi Kitano; Yasushi Maeda; Masayo Yamamoto; Rie Izumida
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Article
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1996
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John Wiley and Sons
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English
β 458 KB
## Abstract A proteolytic enzyme, thermolysin, was covalently immobilized to dextran which has a small number of carboxyl groups. Enzymatic synthesis of aspartame derivative (BocβAspβPheβOMe) using the dextranβconjugated thermolysin was carried out in an aqueous polymer twoβphase system, where poly