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Enzyme activity during the growth and aging of human cells in vitro

✍ Scribed by V. J. Cristofalo; N. Parris; David Kritchevsky

Publisher: John Wiley and Sons
Year: 1967
Tongue: English
Weight: 668 KB
Volume: 69
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040690302

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✦ Synopsis

Acid phosphatase, alkaline phosphatase, and lactic dehydrogenase activities have been compared in normal human diploid cell strains and in SVlVtransformed heteroploid cell lines derived from them. A higher level of acid phosphatase activity was observed in diploid cultures derived from adult lung than in cultures derived from fetal lung of similar passage levels. The alkaline phosphatase activity of normal diploid fibroblasts was significantly higher than that of SVdo-transformed cell lines derived from them. Generally, the lactic dehydrogenase activities of all these cell cultures were similar.

Human diploid cells in culture "age," in the sense that their ability to proliferate decreases with time during serial subcultivation. Evaluation of the activities of these three enzymes during the "aging" process showed that, although alkaline phosphatase and lactic dehydrogenase activities were similar in "young" and "senescent" cells, acid phosphatase showed a small but significant increase in the senescent cells.

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