✦ LIBER ✦

Effect of donor age on the growth in vitro of cells obtained from human trabecular bone

✍ Scribed by Dr. Carol E. Evans; Charles S. B. Galasko; Carol Ward

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 318 KB
Volume: 8
Category: Article
ISSN: 0736-0266
DOI: 10.1002/jor.1100080212

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The proliferation of human bone‐derived cells (BDCs) was assessed in vitro, using [^3^H]thymidine incorporation and cell counting in a haemacytometer. The cells were cultured from human trabecular bone from 87 patients aged 2–88 years. The in vitro growth of these cells was unaffected by the chronological age of the donor. However, the cell number at confluence was shown to decrease with increasing donor age, this trend being most marked after 60 years of age. Other assays of the metabolic efficiency of the BDCs, namely, total protein, osteocalcin, and alkaline phosphatase synthesis, did not show any change with increasing donor age. These results suggest that while the ability of individual cells to divide and to perform specific synthetic activities is unimpaired with increasing age, other subtler changes may occur, leading to a decrease in the bone's osteogenic capacity.

📜 SIMILAR VOLUMES

Effect of growth hormone on in vitro ost

Effect of growth hormone on in vitro osteogenesis and gene expression of human osteoblastic cells is donor-age-dependent

✍ Grasiele E. Crippa; Marcio M. Beloti; Cristina R. Cardoso; João S. Silva; Adalbe 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 173 KB 👁 2 views

## Abstract It has been demonstrated that the effect of GH on bone tissue is reduced with aging. In this study we tested the hypothesis that the action of GH on osteoblastic cells is donor‐age‐dependent by investigating the effect of GH on the development of osteoblastic phenotype in cultures of ce

Effect of bone proteins on human prostat

Effect of bone proteins on human prostate cancer cell lines in vitro

✍ Hullinger, Thomas G.; McCauley, Laurie K.; DeJoode, Melanie L.; Somerman, Martha 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 290 KB 👁 2 views

## Background: Despite the high incidence and serious consequences of skeletal metastasis in prostate cancer patients, the mechanisms involved in establishing secondary lesions in bone are not well-understood. in this study, the role of the mineralized bone matrix in the process of skeletal metasta

The effect of carbon dioxide on human re

The effect of carbon dioxide on human red cells in vitro

✍ Brown, E. A. 📂 Article 📅 1957 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 122 KB 👁 1 views

Effect of endothelial cell conditioned m

Effect of endothelial cell conditioned medium on the growth of human bone marrow fibroblasts

✍ Giovanna Bianchi Scarrà; Paola Fiorentini; Giovanni Garbarino; Graziella Bellone 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 420 KB 👁 1 views

Effects of age and gender on WNT gene ex

Effects of age and gender on WNT gene expression in human bone marrow stromal cells

✍ Longxiang Shen; Shuanhu Zhou; Julie Glowacki 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 219 KB 👁 2 views

## Abstract WNT signaling pathways play important roles in the behavior of human bone marrow stromal cells. Although WNT expression has been examined in human bone marrow stromal cells (hMSCs) with limited numbers of subjects or from commercial sources, there are conflicting results on WNT gene exp

Effect of human leukocyte interferon on

Effect of human leukocyte interferon on the growth of human osteosarcoma cells in tissue culture

✍ Hans Strander; Stefan Einhorn 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 396 KB 👁 1 views

## Abstract Nine osteosarcoma cell lines, originally developed from six osteosarcoma tumours in five patients, and two cell lines of non‐tumour origin (glia and fibro‐blast) were grown __in vitro__ in the presence of human leukocyte interferon (L‐IF). L‐IF exerted a dose‐dependent inhibition of gro