An efficient route for the synthesis of Gal ~I-3GalNAc~ l -O-Serine derivative was developed using a novel [31-3 galactosidase obtained from Bacillus circulans. By a transglycosylation reaction with pNP-~-Gal 2 as a donor and GalNAc~.I-O-Z-Ser-OAII 4 as an acceptor, GaI!31-3GalNAca-I-O-Z-Ser-OAII 5
Enzymatic synthesis of β-galactosyldipeptides and of β-1,3-digalactosylserine derivatives using β-galactosidase
✍ Scribed by Sandra Attal; Sylvie Bay; Danièle Cantacuzene
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- French
- Weight
- 578 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0040-4020
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Disaccharide-peptide conjugates were obtained in yields of 30-50% from o-nitrophenyl beta-D-galactopyranoside by employing beta-D-galactosidase from E. coli as catalyst. Two series of beta-D-galactosyldipeptides were examined as galactosyl acceptors. They both contain an L-serine residue beta-linked
The title trisaccharide-serine conjugate 1 constituting the linkage region between glycoaminoglycaa and I~otein in proteoglycan, was synthesized via a Ixisacchm~k p-nitrophenyl (PNP) glycoside prepared by stepwise enzymatic transglycosidation to XyI-PNP with a ~-D-galactosidase. In the second tnmsgl