Synthesis of Galβ1-3GlcNAc and Galβ1-3GlcNAcβ-SEt by an enzymatic method comprising the sequential use of β-galactosidases from bovine testes andEscherichia coli
✍ Scribed by Lars Hedbys; Elisabet Johansson; Klaus Mosbach; Per-Olof Larsson; Alf Gunnarsson; Sigfrid Svensson; Hans Lönn
- Publisher
- Springer US
- Year
- 1989
- Tongue
- English
- Weight
- 483 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1573-4986
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The title trisaccharide-serine conjugate 1 constituting the linkage region between glycoaminoglycaa and I~otein in proteoglycan, was synthesized via a Ixisacchm~k p-nitrophenyl (PNP) glycoside prepared by stepwise enzymatic transglycosidation to XyI-PNP with a ~-D-galactosidase. In the second tnmsgl
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The tetrasaccharide beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----6)-alpha-D- Manp-(1----6)-beta-D-Manp-OR (2) and the pentasaccharide beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-beta-D- GlcpNAc-(1----6)-alpha-D-Manp-(1----6)-beta-D-Manp-OR (3), where R = (CH2)8COOMe, have been prepared by using combin