Synthesis of a tetrasaccharide acceptor for use in the assay of UDP-GlcpNAc:β-d-Galp-(1→4)-β-d-GlcpNAc (GlcNAc to Gal) β(1→3)-N-acetylglucosaminyltransferase activity and the pentasaccharide product that would be formed by its enzymatic glycosylation
✍ Scribed by Geeta Srivastava; Ole Hindsgaul
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 809 KB
- Volume
- 224
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The tetrasaccharide beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----6)-alpha-D- Manp-(1----6)-beta-D-Manp-OR (2) and the pentasaccharide beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-beta-D- GlcpNAc-(1----6)-alpha-D-Manp-(1----6)-beta-D-Manp-OR (3), where R = (CH2)8COOMe, have been prepared by using combined chemical and enzymic procedures. Structure 2 is a substrate for UDP-GlcpNAc:beta-D-Galp-(1----4)- beta-D-GlcpNAc (GlcNAc to Gal) beta(1----3)-N-acetylglucosaminyltransferase, and 3 would be the product of its action. Antibodies raised against 3 are intended for use in an ELISA assay that would quantitate the enzymic conversion of immobilized 2 into 3.