L-[1.2-13C2, 15N]Serine was prepared from [1,2-13C2, 15N]glycine on a gram scale by the use of the enzyme serine hydroxymethyltransferase. The reaction was monitored by 13C-NMR spectroscopy. This is the first simultaneously 13C- and 15N-labelled serine isotopomer so far reported. Part of the product
Enzymatic synthesis of radio-labeled selenocystine from labeled serine and selenide
✍ Scribed by Gregory L. Dilworth
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- French
- Weight
- 173 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
Selenocysteine can be formed enzymically from O‐acetylserine and sodium selenide by O‐acetylserine sulfhydrylase. The preparation of O‐acetylserine from serine and its conversion to selenocystine are described. These reactions can be used to synthesize [^14^C], [^3^H], or [^75^Se]selenocystine.
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