Enzymatic Syntheses of C 14 -Labeled Uridine Diphosphoglucose, Galactose 1-Phosphate, and Uridine Diphosphogalactose 1
โ Scribed by Anderson, E. P.; Maxwell, Elizabeth S.; Burton, Robert Main
- Book ID
- 126939669
- Publisher
- American Chemical Society
- Year
- 1959
- Tongue
- English
- Weight
- 617 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0002-7863
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๐ SIMILAR VOLUMES
A new sensitive method is described for glucose l-phosphate analysis. The key reaction is the pyrophosphorolysis of UDP-glucose catalyzed by uridine Y-diphosphoglucose pyrophosphorylase. The reaction product, ['4C]UDP-giucose, is separated from [W]UTP by adsorbing [W]UTP selectively onto polyethylen
The sequential enzyme assay as previously described has been used to study various effects on the three enzymes in human red cells involved in the phosphorylation of galactose : galactokinase, galactose-1-phosphate uridyl transferase and uridine diphospho-galactose-4-epimerase. 1. Enzyme activities