✦ LIBER ✦
Enzyme kinetics in mammalian cells. III. Regulation of activities of galactokinase, galactose-1-phosphate uridyl transferase and uridine diphosphogalactose-4-epimerase in human erythrocytes
✍ Scribed by Helene Z. Hill
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 874 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
The sequential enzyme assay as previously described has been used to study various effects on the three enzymes in human red cells involved in the phosphorylation of galactose : galactokinase, galactose-1-phosphate uridyl transferase and uridine diphospho-galactose-4-epimerase.
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Enzyme activities in undiluted lysates appear to reflect the respective activities in whole cells.
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Added extracellular Gal-1-P, G-1-P, UDPGal and UDPG do not affect enzyme activities in whole cells.
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The kinase and transferase enzymes do not appear to be associated with the membrane fraction of the red cells.