Enzymatic reaction kinetic: Comparison in an organic solvent and in supercritical carbon dioxide

✍ Scribed by T. Dumont; D. Barth; C. Corbier; G. Branlant; M. Perrut

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 453 KB
• Volume: 40
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260400218

✦ Synopsis

Myristic acid esterification has been performed by an immobilized lipase from Mucor Miehei both in n-hexane and in supercritical carbon dioxide (SCC02). The enzyme is stable in SCC02 at 15 MPa and 323 K. The reaction rate is influenced by the concentration of water and by the reaction medium composition. A reaction mechanism is proposed, and kinetic parameters are determined at 12.5 MPa and 313 K. Maximum velocity appears 1.Bfold higher in SCC02 than in n-hexane; however, as solubility of myristic acid is greater in n-hexane, it is not yet definitively clear that the supercritical medium is more favorable than the classical organic solvent for this type of enzyme reaction.