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Enzymatic labelling of carcinoembryonic antigen with iodine-125

✍ Scribed by Andrzej Siuda; Barbara Lucka; Krystyna Russin


Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
385 KB
Volume
18
Category
Article
ISSN
0022-2135

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✦ Synopsis


Abstract

Carcinoembryonic antigen /CEA/ was labelled with iodine‐125 using lactoperoxidase. The radiochemical yield of /^125^I/CEA was about 25% at the specific activity of about 30μCi/μg /1.1MBq/μg/. The percentage binding of /^125^I/CEA with specific anti‐CEA serum was determined in lyophilized samples during storage. The obtained /^125^I/CEA preparations can be utilized in radioimmunoassay kits for at least two months.


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