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Enzymatic determination of alcohol mixtures at the nanogram level by the stopped-flow technique

✍ Scribed by Eva Förster; Manuel Silva; Matthias Otto; Dolores Pérez-Bendito

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
692 KB
Volume
274
Category
Article
ISSN
0003-2670

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✦ Synopsis

AIlStraCt

A sensltlve kmetlc enzymatic method for the mdnndual and Joint determmatlon of lower straight-cham alcohols IS reported The method IS based on reaction of the alcohols Hrlth the alcohol dehydrogenase-mcotmamlde adenme dmucleotlde system, which IS momtored by measurmg the mltlal rate of change of the absorbance or fluorescence mtenslty of the reduced coenzyme formed by usmg the stopped-flow techmque Methanol, ethanol, propanol and butanol can thus be determmed over the range 3 0 X 10-*-l 0 X 10m6 M with a relatrve standard devtation of ca 12-3 8% Spectrofluonmetnc measurements are better suded to the mdlvldual determmatlons, whereas spectrophotometnc measurements are more useful for resolvmg alcohol mmtures usmg the Kahnan filter algonthm Mixtures of methanol-butanol, ethanol-butanol and methanol-propanol at the submxromolar level were successfully resolved v&h errors of less than 10%

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