An enzymatic method for D-carnitine determination using the enzyme D-carnitine dehydrogenase is described. The assay is based on the amplified signal produced during NAD Ψ cycling in the presence of a tetrazolium salt and using phenazine methosulfate as electron carrier. Optimum assay conditions wer
Enzymatic cycling assay for phenylpyruvate
β Scribed by Arthur J.L. Cooper; Lucas K.H. Leung; Yasuhisa Asano
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 628 KB
- Volume
- 183
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Methods for measuring total glutathione are described. These are based on the ability of glutathione and glutathione reductase (EC 1.6.4.2.) to catalyze the oxidation of NADPH by Ellman's reagent. Except for highest glutathione levels, NADP+ rather than the reduced Ellman compound is measured. For i
unc-25 in muscle cells (8) and GABAergic neurons (10), respectively. Without TSA, probe detection resulted in no hybridization signal. When TSA was performed, expression of unc-49 and unc-25 was detected in body wall muscles (Fig. ) and neurons, respectively. In conclusion, TSA is useful in the enh