A solid phase DNA sequencing method for non-radioactive detection of single base changes without the need for electrophoresis is presented. The concept relies on the detection of DNA polymerase activity by an enzymatic luminometric inorganic pyrophosphate detection assay (P. NyrΓ©n, 1987, Anal. Bioch
Enzymatic Chemiluminescence Assay for Inorganic Pyrophosphate
β Scribed by Vuokko Jansson; Kristian Jansson
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 123 KB
- Volume
- 304
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
unc-25 in muscle cells (8) and GABAergic neurons (10), respectively. Without TSA, probe detection resulted in no hybridization signal. When TSA was performed, expression of unc-49 and unc-25 was detected in body wall muscles (Fig. ) and neurons, respectively.
In conclusion, TSA is useful in the enhancement of in situ hybridization signals in C. elegans.
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