✦ LIBER ✦

Enzymatic assay for glutathione

✍ Scribed by Jacqueline E. Brehe; Helen B. Burch

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 553 KB
Volume: 74
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90323-7

No coin nor oath required. For personal study only.

✦ Synopsis

Methods for measuring total glutathione are described. These are based on the ability of glutathione and glutathione reductase (EC 1.6.4.2.) to catalyze the oxidation of NADPH by Ellman's reagent. Except for highest glutathione levels, NADP+ rather than the reduced Ellman compound is measured. For intermediate sensitivity (2 x lo-'* mol) NADP is measured stoichiometrically by conversion to NADPH and determination of fluorescence. For smaller amounts (lo-r4 mol) the NADP+ generated is amplified by enzymatic cycling. These procedures have been tested extensively on kidney and are probably applicable to tissues in general.

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