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Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro

โœ Scribed by Simon, Marcia; Green, Howard


Book ID
122504919
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
954 KB
Volume
40
Category
Article
ISSN
0092-8674

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๐Ÿ“œ SIMILAR VOLUMES


Enzymatic cross-linking of involucrin an
โœ Simon, Marcia; Green, Howard ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 954 KB

A transglutaminase-catalyzed cross-linking process characteristic of keratinocytes leads to the formation of the insoluble corneocyte envelope. The essentials of this process take place in vitro in a reconstituted system derived from subcellular fractions. A particulate fraction containing membrane-

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## Abstract The enzyme peroxidase, a substrate (hydrogen donor), and hydrogen peroxide aggregated and polymerized soluble proteins included in the reaction mixture. Gel filtration and acrylamide disk gel electrophoresis revealed newly formed dimers, trimers, and higher protein polymers. Some of the