Enzymatic ATP formation from ADP and phosphoramidate
โ Scribed by H.Leigh Auleb; Michael J. Dowler; Henry I. Nakada
- Book ID
- 118849771
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 323 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-291X
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๐ SIMILAR VOLUMES
The title compound was prepared by a three-stage enzymatic procedure consisting of (i) RNA hydrolysis to a mixture of ribonucleosides using intact myceUum of Spicaria violacea, (ii) transribosylation of exogenous adenine employing whole cells of Escherichia coil as a biocatalyst, and (iii) conversio
An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretic