Enzymatic synthesis of ATP from RNA and adenine

✍ Scribed by V. N. Barai; A. I. Zinchenko; L. M. Zalashko; L. A. Eroshevskaya; I. A. Mikhailopulo

• Book ID: 104633556
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 229 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00129385

✦ Synopsis

The title compound was prepared by a three-stage enzymatic procedure consisting of (i) RNA hydrolysis to a mixture of ribonucleosides using intact myceUum of Spicaria violacea, (ii) transribosylation of exogenous adenine employing whole cells of Escherichia coil as a biocatalyst, and (iii) conversion of formed adenosine into ATP by the enzymes of alcohol fermentation and the kinases extracted from baker's yeast.