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Entrapment of lipase in silica glass by the sol-gel method and its esterification activity in organic media

✍ Scribed by Koei Kawakami; Shigehiro Yoshida

Publisher: Springer-Verlag
Year: 1994
Tongue: English
Weight: 396 KB
Volume: 8
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00154318

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✦ Synopsis

Silica glass-entrapped lipase was prepared by the sol-gel method using tetramethoxysilane, and its esterification activity in n-hexane was examined for isoamylbutyrate formation. The hydrogel preparation containing a large amount of water exhibited enough activity. Although the activity of xerogel-entrapped lipase drastically decreased probably due to shrinkage of the gel matrix, the lyophilized gel retained much higher activity than the air-dried gel.

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