Sol-gel entrapment of lipase using a mixture of tetramethoxysilane and methyltrimethoxysilane as the alkoxide precursor: Esterification activity in organic media

✍ Scribed by Koei Kawakami; Shigehiro Yoshida

• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 282 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00159232

✦ Synopsis

The sol-el fi method, usin 6 a mixture of tetramethoxysilane (TMOS) and methyltrimet ox P silane (MTrM S) as the silicon alkoxide precursor, has been applied to entrapment o lipase catalyzing esterification reactions in organic media. Increasing molar ratio of MTrMOS to TMOS led to formation of opaque and non-shrinkable gels and resulted in an increase in the esterification activity of lipase. The hybrid gel-entrapped lipase based on [MTrMOS]/[TMOS] ratio of 4 exhibited almost equal activity with those of the de higher activity K osited lipase on different supports at 35"C, and retained ca. ten times t an the deposited counterparts at 65 %.