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Enhancement of protein synthesis with sodium ion in a cell-free system from Escherichia coli

✍ Scribed by Dong-Myung Kim; You-Eil Kim; Cha-Yong Choi

Book ID
113397958
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
329 KB
Volume
82
Category
Article
ISSN
0922-338X

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led