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Cell-free system derived from heat-shocked Escherichia coli: Synthesis of enzyme protein possessing higher specific activity

✍ Scribed by Norihiro Nishimura; Yoshihisa Kitaoka; Mitsuru Niwano


Book ID
113397640
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
647 KB
Volume
79
Category
Article
ISSN
0922-338X

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led