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Comparative evaluation of two cell-free protein synthesis systems derived from Escherichia coli for genetic code reprogramming

✍ Scribed by Lee, Ki Baek; Kim, Ho-Cheol; Kim, Dong-Myung; Kang, Taek Jin; Suga, Hiroaki


Book ID
122042163
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
589 KB
Volume
164
Category
Article
ISSN
0168-1656

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led