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Oxalate improves protein synthesis by enhancing ATP supply in a cell-free system derived from Escherichia coli

✍ Scribed by Dong-Myung Kim; James R. Swartz


Book ID
110226520
Publisher
Springer Netherlands
Year
2000
Tongue
English
Weight
66 KB
Volume
22
Category
Article
ISSN
0141-5492

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Expression of functional Candida antarct
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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led