Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase
β Scribed by Mehmet Inan; Dinesh Aryasomayajula; Jayanta Sinha; Michael M. Meagher
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 210 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The biologically active protein known as human granulocyte colony stimulating factor (GβCSF) can be efficiently secreted from the transformed __GS115 Pichia pastoris__ (__GS115/pPIC9/GβCSF__), which contains an Ξ±βmatingβfactor prepro signal sequence and an alcohol oxidase I promoter, bo
## Abstract A method combining fluorescence activated cell sorting (FACS) and DNA microarray assisted clone identification was developed and termed GenomeβScale Analysis of Library Sorting (GALibSo). Genes enhancing the production of secreted heterologous proteins in __Pichia pastoris__ were identi
## Abstract The translation of highly repetitive gene sequences is often associated with reduced levels of protein expression and may be prone to mutational events. In this report, we describe a modified concatemerization strategy to construct a gene with enhanced sequence diversity that encodes a