The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high levels under the control of the methanol-inducible alcohol oxidase 1 (AOX1) promoter. Accurate regulation of the methanol concentration in P. pastoris cultures is necessary to maintain induction, while preventi
โฆ LIBER โฆ
Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris
โ Scribed by Narendar K. Khatri; Frank Hoffmann
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 186 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0006-3592
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An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut + expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(H c )], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities. The model describes