𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High level expression of heterologous proteins in methylotrophic yeast Pichia pastoris

✍ Scribed by Dr. K. Sreekrishna; Rica H. B. Potenz; John A. Cruze; William R. McCombie; Kathryn A. Parker; Lynn Nelles; Paul K. Mazzaferro; Kathy A. Holden; Roger G. Harrison; Phyllis J. Wood; Doris A. Phelps; Cindi E. Hubbard; Motohiro Fuke


Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
912 KB
Volume
28
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Expression of heterologous proteins in P
✍ Rachel Daly; Milton T. W. Hearn πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 373 KB πŸ‘ 1 views

The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of recombinant proteins has become increasing popular in recent times. P. pastoris is easier to genetically manipulate and culture than mammalian cells and can be grown to high cell densities. Equally importa

Fermentation strategies for improved het
✍ Feng Hong; Nina Q. Meinander; Leif J. JΓΆnsson πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 206 KB πŸ‘ 1 views

## Abstract Improved expression of recombinant laccase by __Pichia pastoris__ carrying the __lcc1__ cDNA isolated from __Trametes versicolor__ was achieved by optimization of the cultivation conditions in a fermentor equipped with a methanol sensor system. The results indicated that the activity ob

Recombinant Production of Human Microsom
✍ Matthias Dietrich; Lisa Grundmann; Katja Kurr; Laura Valinotto; Tanja Saussele; πŸ“‚ Article πŸ“… 2005 πŸ› John Wiley and Sons 🌐 English βš– 223 KB πŸ‘ 1 views

## Abstract __Microsomal cytochrome P450 monooxygenases of groups 1–3 are mainly expressed in the liver and play a crucial role in phase 1 reactions of xenobiotic metabolism. The cDNAs encoding human CYP2D6 and human NADPH‐P450 oxidoreductase (CPR) were transformed into the methylotrophic yeast__ P