Enhancement of L-glutamate oxidase production in liquid fermentation ofStreptomycessp. by calcium addition

✍ Scribed by Q. -S. Li; J. -J. Zhong

• Book ID: 104651703
• Publisher: Springer-Verlag
• Year: 1996
• Tongue: English
• Weight: 263 KB
• Volume: 10
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00174232

✦ Synopsis

The effect of calcium ions on production of extracellular L-glutamate oxidase (GluOx) in liquid fermentation of Streptomyces sp. N1 was investigated. By supplementing a relatively large amount of Ca2+ (15-40 n-M) to the medium, the GluOx production was significantly enhanced, although the microbial growth was inhibited to some degree. For the first time, the highest production (i.e. 6.5 U/ml) and productivity (i.e. 0.25 U/ml/h) of GluOx as ever reported (i.e., 1.2 U/g medium after 144 h solid state fermentation and 0.59 U/ml after 96 h liquid fermentation) were achieved with addition of 30 mM Ca2+.