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Enhancement of hepatitis-B surface-antigen expression by 5-azacytidine in a hepatitis-B-virus-transfected cell line

✍ Scribed by Eiji Miyoshi; Junichi Fujii; Norio Hayashi; Keiji Ueda; Takahiro Towata; Hideyuki Fusamoto; Takenobu Kamada; Naoyuki Taniguchi

Publisher: John Wiley and Sons
Year: 1992
Tongue: French
Weight: 495 KB
Volume: 52
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910520124

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✦ Synopsis

The human hepatoblastoma-derived cell line HB6 I I secretes hepatitis-B surface antigen (HBsAg) and hepatitis-B e antigen (HBeAg) into the medium. Hepatitis-B-virus(HBV) DNA integrated into the cellular genome was found to be hypermethylated. When the cells were treated with 5-azacytidine for 3 days, the level of HBsAg in the medium increased, while the level of HBeAg remained constant. The level of alphafetoprotein (AFP) decreased with the 5-azacytidine treatment. Southern blot analysis of DNA digested with Hpall or Mspl showed that 5-azacytidine treatment resulted in hypomethylation of the integrated HBV DNA, suggesting that 5-azacytidine increased HBsAg production in the cells through hypomethylation of the HBV genomic DNA.

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