Engineering Zinc Finger Proteins using Accessory Binding Modules
โ Scribed by Trenshaw, Kathryn; Kim, Younghoon; Yee, Nathan; Wang, Peiyi; Schroeder, Charles
- Book ID
- 119209708
- Publisher
- Biophysical Society
- Year
- 2012
- Tongue
- English
- Weight
- 43 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0006-3495
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๐ SIMILAR VOLUMES
## Abstract Increasing the yield of therapeutic proteins from mammalian production cell lines reduces costs and decreases the time to market. To this end, we engineered a zinc finger protein transcription factor (ZFP TF) that binds a DNA sequence within the promoter driving transgene expression. Th
Artificial transcription factors based on modified zinc-finger DNA-binding domains have been shown to activate or repress the transcription of endogenous genes in multiple organisms. Advances in both the construction of novel zinc-finger proteins and our understanding of the characteristics of a pro