The hazards posed to the environment by the accidental release of baculovirus expression vectors can be put into perspective by the results obtained from experiments in which AcNPV was released deliberately into the field (Bishop et al., 1992). Polyhedrin positive viruses will persist in soil and on
Engineering aspects of insect cell suspension culture: a review
โ Scribed by J. Wu; G. King; A. J. Daugulis; P. Faulkner; D. H. Bone; M. F. A. Goosen
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 665 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1432-0614
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โฆ Synopsis
The development of insect cell suspension culture techniques for the production of insect pathogenic viruses and recombinant proteins has been reviewed, with an emphasis on process scale-up and reactor design considerations. The problems of culture media cost and insect cell shear sensitivity have also been addressed.
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Stable transformed insect cell lines have been used for producing many highly processed heterologous proteins. Current research has focused on development of new expression and selection systems, and enhancement of vector stability. Defining the variation of modification and processing capabilities