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Engineered Biosynthesis of Novel Polyketides: Dissection of the Catalytic Specificity of the act Ketoreductase

✍ Scribed by Fu, Hong; Ebert-Khosla, Susanne; Hopwood, David A.; Khosla, Chaitan


Book ID
126895968
Publisher
American Chemical Society
Year
1994
Tongue
English
Weight
1003 KB
Volume
116
Category
Article
ISSN
0002-7863

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DEBS 1 + TE is a recombinant modular polyketide synthase (PKS) in which the first two biosynthetic modules of the 6-deoxyerythronolide B synthase are linked to the thioesterase domain normally found at the C-terminus of DEBS 3. Incubation of DEBS 1 + TE with propionyl-CoA, methylamalonyl-CoA, and NA