Endotoxin activity measured by limulus assay
โ Scribed by E. Haight-Ponce; H. Endo; H. Horiuchi
- Book ID
- 111270724
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 547 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1600-4469
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentr
MATERIALS AND METHODS ## Reagents. Lyophilized Litnultrs amebocyte lysate (Pyrogent. purchased from Mallinckrodt (St. Louis, MO.) was used in all experiments. The redissolved reagent was kept frozen at -20ยฐC in small aliquots for no longer than 4 weeks.