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End-to-end distance distribution in bradykinin observed by Förster resonance energy transfer

✍ Scribed by Eduardo Sérgio de Souza; Izaura Yoshico Hirata; Luiz Juliano; Amando Siuiti Ito


Book ID
117480591
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
393 KB
Volume
1474
Category
Article
ISSN
0304-4165

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We describe a new method to recover the distribution of distances between two sites on flexible molecules, which requires only steady state measurements of fluorescence intensities. We used collisional quenching of the donor fluorescence to vary the characteristic Wrster distance (R,) for energy tra