End-to-end distance distributions of flexible molecules from steady state fluorescence energy transfer and quenching-induced changes in the Förster distance
✍ Scribed by Ignacy Gryczynski; Wieslaw Wiczk; Michael L. Johnson; Joseph R. Lakowicz
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 660 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0009-2614
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✦ Synopsis
We describe a new method to recover the distribution of distances between two sites on flexible molecules, which requires only steady state measurements of fluorescence intensities. We used collisional quenching of the donor fluorescence to vary the characteristic Wrster distance (R,) for energy transfer. The measured transfer efficiencies for each value of R,, provide different samples of the distance distribution. The R,dependent transfer efficiencies, when analyzed by non-linear least squares, were found to reliably determine the distance distribution, as shown by evaluation of the uncertainties in the distribution and by agreement with the frequency-domain method (Chem. Phys.