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Conformational analysis of galanin using end to end distance distribution observed by Förster resonance energy transfer

✍ Scribed by T. Kulinski; Anders B. A. Wennerberg; R. Rigler; Stephen W. Provencher; M. Pooga; Ü. Langel; Tamas Bartfai


Book ID
105946989
Publisher
Springer
Year
1997
Tongue
English
Weight
621 KB
Volume
26
Category
Article
ISSN
1432-1017

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We describe a new method to recover the distribution of distances between two sites on flexible molecules, which requires only steady state measurements of fluorescence intensities. We used collisional quenching of the donor fluorescence to vary the characteristic Wrster distance (R,) for energy tra