Articles In The Fields Of Biochemistry And Physiology, Cell Biology, Developmental Biology, Ecology, Evolution, Genetics, Immunology, Molecular Biology, Neuroscience, Microbiology And Virology, Plant Science, Structural Biology, Science And Society. Includes Index.
Encyclopedia of Life Sciences || Gel Electrophoresis of RNA in Denaturing Formaldehyde Agarose Gels
β Scribed by Southern, Edwin M
- Book ID
- 120423466
- Publisher
- John Wiley & Sons, Ltd
- Year
- 2003
- Tongue
- English
- Weight
- 87 KB
- Category
- Article
- ISBN-13
- 9780470015902
No coin nor oath required. For personal study only.
β¦ Synopsis
Articles In The Fields Of Biochemistry And Physiology, Cell Biology, Developmental Biology, Ecology, Evolution, Genetics, Immunology, Molecular Biology, Neuroscience, Microbiology And Virology, Plant Science, Structural Biology, Science And Society. Includes Index.
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This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal-or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert low conductivity buffer. Following electrophoresis, the gel is stained with a sensitive silver
The possibility of using fomaldehyde as a denaturant for DNA in agarose-or polyacrylamide-gel electrophoretic systems was studied. It was found possible to fractionate and determine the molecular weights of denatured DNA using calibration curves constructed with single-stranded DNA fragments of know