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Electrophoresis of denatured DNA in gels containing formaldehyde

✍ Scribed by M. Yaneva; J. Mladenova; G. Dessev

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 400 KB
Volume: 77
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90245-7

No coin nor oath required. For personal study only.

✦ Synopsis

The possibility of using fomaldehyde as a denaturant for DNA in agarose-or polyacrylamide-gel electrophoretic systems was studied. It was found possible to fractionate and determine the molecular weights of denatured DNA using calibration curves constructed with single-stranded DNA fragments of known molecular weights.

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