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Agarose gel electrophoresis of denatured RNA with silver staining

✍ Scribed by Rose N. Skopp; Leslie C. Lane

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 627 KB
Volume: 169
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90263-1

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✦ Synopsis

This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal-or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert low conductivity buffer. Following electrophoresis, the gel is stained with a sensitive silver stain. The method produces sharp, well-resolved bands and yields accurate RNA size estimates. Because of its sensitivity and simplicity, it is suitable for routine laboratory use. 8 1988 Academic Press. inc.

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