Enantioseparation of chiral tropa alkaloids by means of cyclodextrin-modified microemulsion electrokinetic chromatography

✍ Scribed by Yaser Bitar; Ulrike Holzgrabe

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 384 KB
• Volume: 28
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200700008

✦ Synopsis

Abstract

CD‐modified microemulsion EKC as a CE technique has been applied to the chiral separation of atropine, scopolamine, ipratropium and homatropine. Enantioseparations of these tropa alkaloids were optimized by using a standard oil‐in‐water (O/W) microemulsion and varying the nature and concentration of CD additives as well as of the organic modifier (methanol, 2‐propanol or ACN) whilst keeping the applied voltage of 15 kV and capillary temperature of 30°C constant. The standard (O/W) microemulsion BGE solution consisted of 0.8% w/w octane, 6.6% w/w 1‐butanol, 2.0% w/w SDS and 90.6% w/w 10 mM sodium tetraborate buffer (pH 9.2). Enantioseparations with high resolution and short migration times of all tropa alkaloids were achieved by using heptakis(2,3‐di‐O‐methyl‐6‐O‐sulfo)‐β‐CD and sulfated β‐CD in the microemulsion BGE and were superior to corresponding CD‐modified CE methods.