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Enantiometric separation of di- and tri-peptides with chiral fluorescence labeling reagents by liquid chromatography

✍ Scribed by Yi-Ming Liu; Jian-Rong Miao; Toshimasa Toyo'oka

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 410 KB
Volume: 314
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(95)00270-a

No coin nor oath required. For personal study only.

✦ Synopsis

Stereoisomers

of di-and tri-peptides were separated on C,, columns after their pre-column derivatization with the chiral fluorescence labeling reagent, R( -

The labeling reaction was usually carried out with 1 pg of peptide sample dissolved in water in basic solution (0.1% (v/v) triethylamine) at 60°C for 10 min. No observable racemization occurred during the derivatization.

All of the four stereoisomers of the peptides tested could be resolved with mixtures of water/acetonitrile/methanol containing 0.05% (v/v) trifluoroacetic acid (TFA) as the mobile phases. The eluate was monitored by a fluorescence detector with the excitation and emission wavelengths set at 450 nm and 560 nm, respectively. This method is useful for enantiospecific quantitations and quality assurance tests of peptides, particularly in the cases where only minute amounts of samples are available.

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