Enantiomeric separation by capillary electrophoresis of di- and tri-peptides derivatized with 9-fluorenylmethyl chloroformate using vancomycin as chiral selector

✍ Scribed by Hong Wan; Lars G. Blomberg

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 566 KB
• Volume: 8
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1996)8:5<339::aid-mcs3>3.0.co;2-2

✦ Synopsis

Enantiomeric separations of chiral di-and tri-peptides derivatized with 9-fluorenylmethyl chloroformate were carried out by capillary electrophoresis using vancomycin as a chiral selector. The mobilities of vancomycin were determined in phosphate and HEPES buffer by means of a factorial design. The zero mobility of vancomycin was found to be, to a small extent, dependent on buffer type, buffer concentration, and the presence of organic modifier. Application of a pH above zero mobility of vancomycin resulted in highly efficient separation of 15 di-and tri-peptides in less than 12 min. The influence of 2-propanol, buffer types, and sodium dodecyl sulfate on selectivity and efficiency was also investigated. o 1996