Enantiomeric separation of dihydroxyphenyl-alanine (DOPA), methyldihydroxyphenylalanine (MDOPA) and hydrazinomethyldihydroxyphenyl-alanine (CDOPA) by using capillary electrophoresis with sulfobutyl ether-β-cyclodextrin as a chiral selector
✍ Scribed by Milada Dolezalová; Salvatore Fanali
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 116 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Enantiomeric separation of dihydroxyphenylalanine (DOPA), methyldihydroxyphenylalanine (MDOPA) and hydrazinomethyldihydroxyphenylalanine (CDOPA) by using capillary electrophoresis with sulfobutyl ether-b-cyclodextrin as a chiral selector Capillary electrophoresis (CE) was successfully applied to the enantiomer resolution of racemic structurally related compounds, namely dihydroxyphenylalanine (DOPA), methyldihydroxyphenylalanine (MDOPA) and hydrazinomethyldihydroxyphenylalanine (CDOPA). The chiral resolution was performed in an untreated fused-silica capillary by using a phosphate buffer at pH 2.5 or 3.0 supplemented with sulfobutylated b-cyclodextrin (SBE-CD). Resolution was strongly influenced by the concentration of the chiral selector added to the background electrolyte. In fact, 2±5 mM of SBE-CD enabled the resolution of DOPA and MDOPA enantiomers, while CDOPA optical isomers were resolved by using either 0.5 mM or 6±20 mM of SBE-CD. The latter separation conditions (reversed polarity mode) made it possible to obtain inversion of migration order.