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Elucidation of the ε−θ Subunit Interface of Escherichia coli DNA Polymerase III by NMR Spectroscopy †

✍ Scribed by DeRose, Eugene F.; Darden, Thomas; Harvey, Scott; Gabel, Scott; Perrino, Fred W.; Schaaper, Roel M.; London, Robert E.


Book ID
126417935
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
605 KB
Volume
42
Category
Article
ISSN
0006-2960

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The subunit of DNA polymerase III, the main replicative polymerase of Escherichia coli, has been examined by circular dichroism and by NMR spectroscopy. The polymerase core consists of three subunits: ␣, ⑀, and , with ␣ possessing the polymerase activity, ⑀ functioning as a proofreading exonuclease,